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KMID : 0360220090500081247
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Journal of the Korean Ophthalmological Society
2009 Volume.50 No. 8 p.1247 ~ p.1253
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Effects of Oxidative Stress and Antioxidant on the Expression of Heme Oxygenase-1 in Human RPE
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Kim Young-Jun
Chin Hee-Seung
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Abstract
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Purpose: To evaluate the effects of oxidative stress and antioxidantson heme oxygenase-1 (HO-1) in cultured human retinal pigment epithelial (RPE) cells.
Methods: Cultured RPE cells were challenged with different concentrations of hydrogen peroxide (H2O2), and the HO-1 mRNA level was determined by RT-PCR after 24 hours and 48 hours of incubation independently. Additionally, the HO-1 mRNA level was measured after preincubating RPE cells with N-acetylcystein (NAC) as the antioxidant for 30 minutes and then challenging the cells with H2O2.
Results: The expression of the HO-1 mRNA level increased after H2O2 exposure, and this level was proportional to the increased H2O2 concentration (p<0.05). Expression of the HO-1 mRNA level decreased when the RPE cells were preincubated with NAC (p<0.05).
Conclusions: In human RPE cells, the HO-1 level increased in proportion to the degree of oxidative stress and decreased with exposure to antioxidants. Expression of HO-1 may be helpful in preventing retinal disease, which occurs due to oxidative stresses such as age-related macular degeneration.
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KEYWORD
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Age related macular degeneration, Antioxidant, Heme oxygenase-1, Oxidative stress, Retinal pigment epithelium
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